Optimizing polyethylenimine-based gene transfer into mammalian brain for analysis of promoter regulation and protein function.
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چکیده
منابع مشابه
The Demonstration of Polyethylenimine Mediated Gene Transfer into the Rodent Hypothalamus Results in Persistent Over-Expression and Phenotypic Change
Polyethylenimine (PEI) has been proposed as a non-viral vector, and has been successfully used to transfer reporter genes into the central nervous system (CNS), kidneys, and lungs of adult mice. Neuropeptide Y (NPY) is a peptide expressed in the hypothalamus and is important in the regulation of body weight. Using PEI combined with stereotactic microinjection, we have successfully transferred c...
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عنوان ژورنال:
- Methods in molecular biology
دوره 133 شماره
صفحات -
تاریخ انتشار 2000